首页> 外文OA文献 >Methods, quality control and specimen management in an international multicentre investigation of type 1 diabetes: TEDDY
【2h】

Methods, quality control and specimen management in an international multicentre investigation of type 1 diabetes: TEDDY

机译:1型糖尿病国际多中心研究中的方法,质量控制和标本管理:TEDDY

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

BackgroundThe vast array and quantity of longitudinal samples collected in The Environmental Determinants of Diabetes in the Young study present a series of challenges in terms of quality control procedures and data validity. To address this, pilot studies have been conducted to standardize and enhance both biospecimen collection and sample obtainment in terms of autoantibody collection, stool sample preservation, RNA, biomarker stability, metabolic biomarkers and T-cell viability. Research Design and MethodsThe Environmental Determinants of Diabetes in the Young is a multicentre, international prospective study (n=8677) designed to identify environmental triggers of type 1 diabetes (T1D) in genetically at-risk children from ages 3months until 15years. The study is conducted through six primary clinical centres located in four countries. ResultsAs of May 2012, over three million biological samples and 250 million total data points have been collected, which will be analysed to assess autoimmunity status, presence of inflammatory biomarkers, genetic factors, exposure to infectious agents, dietary biomarkers and other potentially important environmental exposures in relation to autoimmunity and progression to T1D. ConclusionsDetailed procedures were utilized to standardize both data harmonization and management when handling a large quantity of longitudinal samples obtained from multiple locations. In addition, a description of the available specimens is provided that serve as an invaluable repository for the elucidation of determinants in T1D focusing on autoantibody concordance and harmonization, transglutaminase autoantibody, inflammatory biomarkers (T-cells), genetic proficiency testing, RNA lab internal quality control testing, infectious agents (monitoring cross-contamination, virus preservation and nasal swab collection validity) and HbA(1c) testing. Copyright (c) 2013 John Wiley & Sons, Ltd.
机译:背景Young研究中的《糖尿病的环境决定因素》中收集的大量纵向样品在质量控制程序和数据有效性方面提出了一系列挑战。为了解决这个问题,已经进行了初步研究,以在自身抗体收集,粪便样品保存,RNA,生物标志物稳定性,代谢生物标志物和T细胞生存力方面标准化和增强生物样品的收集和样品的获取。研究设计和方法《青年人糖尿病的环境决定因素》是一项多中心的国际前瞻性研究(n = 8677),旨在确定3个月至15岁的有遗传风险的儿童中1型糖尿病(T1D)的环境诱因。该研究是通过位于四个国家的六个主要临床中心进行的。结果截至2012年5月,已收集了超过300万个生物样本和2.5亿个数据点,将进行分析以评估自身免疫状况,炎症性生物标志物的存在,遗传因素,传染源,饮食生物标志物的暴露以及其他潜在的重要环境暴露与自身免疫和发展为T1D有关。结论当处理从多个位置获得的大量纵向样本时,采用了详细的程序来标准化数据协调和管理。此外,提供了可用标本的描述,可作为阐明T1D中决定因素的宝贵资料库,重点关注自身抗体的一致性和协调性,转谷氨酰胺酶自身抗体,炎性生物标志物(T细胞),遗传能力测试,RNA实验室内部质量对照测试,传染原(监测交叉污染,病毒保存和鼻拭子收集有效性)和HbA(1c)测试。版权所有(c)2013 John Wiley&Sons,Ltd.

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号